A conserved tooth resorption mechanism in modern and fossil snakes.

Whether snakes evolved their elongated, limbless bodies or their specialized skulls and teeth first is a central question in squamate evolution. Identifying features shared between extant and fossil snakes is therefore key to unraveling the early evolution of this iconic reptile group. One promising candidate is their unusual mode of tooth replacement, whereby teeth are replaced without signs of external tooth resorption. We reveal through histological analysis that the lack of resorption pits in snakes is due to the unusual action of odontoclasts, which resorb dentine from within the pulp of the tooth. Internal tooth resorption is widespread in extant snakes, differs from replacement in other reptiles, and is even detectable via non-destructive µCT scanning, providing a method for identifying fossil snakes. We then detected internal tooth resorption in the fossil snake Yurlunggur, and one of the oldest snake fossils, Portugalophis, suggesting that it is one of the earliest innovations in Pan-Serpentes, likely preceding limb loss.

Microscopic characterisation of black crusts on different substrates.

Black crusts on different substrates (carbonate and silicate stones, cement-based mortar and carbonate detrital deposits) in urban environments were characterized microscopically by analysing their morphologies and compositions. The objective of this article is to study the interaction between the substrate and the crust and the influence of the environmental conditions on the crust development. On the one hand, the internal structure and morphology of each sample were evaluated with stereo and scanning electron microscopies. On the other hand, the black crust composition was accessed by X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy in backscattered electron mode combined with energy dispersive x-ray spectroscopy. The results of these analytical techniques provided interesting information about the composition and the mineralogical phases present in the collected black crusts. In most of the cases, gypsum was detected as the main component exhibiting different habits. Calcite was also detected in all the evaluated gypsum black crusts; its presence was attributed to different origins. The substrate-crust interaction was also evaluated, contributing to distinguish different crust development processes in relation to the substrate. In carbonate substrates (limestones, mortar and carbonate detrital deposits), it was detected a continuous diffuse boundary related to the replacement of Ca-carbonate by Ca-sulphate, while this boundary was significantly more defined for the granitic stone. This study shows that the substrate, the presence of different construction materials, (e.g. mortars), the motor exhaust particulate substances and the concentrations of atmospheric pollutants, the marine influence as well as biological or other anthropogenic compounds are decisive factors in the development of the black crust. Some ideas about the establishment of conservation strategies are also shown.

Portuguese tin-glazed earthenware from the 17th century. Part 2: A spectroscopic characterization of pigments, glazes and pastes of the three main production centers.

Sherds representative of the three Portuguese faience production centers of the 17th century - Lisbon, Coimbra and Vila Nova were studied with the use of mostly non-invasive spectroscopies, namely: ground state diffuse reflectance absorption (GSDR), micro-Raman, Fourier-transform infrared (FT-IR) and proton induced X-ray (PIXE) or X-ray fluorescence emission (XRF). X-ray diffraction (XRD) experiments were also performed. The obtained results evidence a clear similarity in the pastes of the pottery produced Vila Nova and some of the ceramic pastes from Lisbon, in accordance with documental sources that described the use of Lisbon clays by Vila Nova potters, at least since mid 17th century. Quartz and Gehlenite are the main components of the Lisbon's pastes, but differences between the ceramic pastes were detected pointing out to the use of several clay sources. The spectroscopic trend exhibited Coimbra's pottery is remarkably different, Quartz and Diopside being the major components of these pastes, enabling one to well define a pattern for these ceramic bodies. The blue pigment from the Lisbon samples is a cobalt oxide that exists in the silicate glassy matrix, which enables the formation of detectable cobalt silicate microcrystals in most productions of the second half of the 17th century. No micro-Raman cobalt blue signature could be detected in the Vila Nova and Coimbra blue glazes. This is in accordance with the lower kiln temperatures in these two production centers and with Co(2+) ions dispersed in the silicate matrix. In all cases the white glaze is obtained with the use of tin oxide. Hausmannite was detected as the manganese oxide mineral used to produce the purple glaze (wine color "vinoso") in Lisbon.

Microstructural analysis of Iberian expanded clay aggregates.

This article presents a detailed study of the microstructure of Iberian expanded clay lightweight aggregates (LWA). Other than more commonly used mercury porosimetry (MP) and water absorption methods, the experimental study involves optical microscopy, scanning electron microscopy (SEM), and microtomography (µ-CT). Pore connectivity and how it is deployed are shown to some degree, and the pore size spectrum is estimated. LWA are in general characterized by a dense outer shell up to 200 µm thick, encasing an inner cellular structure of 10-100 times bigger pore size. Aggregate pore sizes may span from some hundreds of nanometers up to over 1 mm, though the range of 1-25 µm is more typical. A noteworthy fraction of these pores is closed, and they are mainly up to 1 µm. It is also shown that macropore spatial arrangement is affected by the manufacturing process. A step forward is given to understanding how the outer shell and the inner pore network influence the mechanical and physical LWA properties, particularly the density and water absorption. The joint consideration of µ-CT and SEM seems to be the most appropriate methodology to study LWA microstructure. MP analysis is likely to distort LWA pore spectrum assessment.